The mechanism of DNA repair by uracil-DNA glycosylase: studies using nucleotide analogues

Rösler A and Panayotou G and Hornby DP and Barlow T and Brown T and Pearl LH and Savva, Renos and Blackburn GM (2000) The mechanism of DNA repair by uracil-DNA glycosylase: studies using nucleotide analogues. Nucleosides, Nucleotides and Nucleic Acids 19 (10-12), pp. 1505-1516. ISSN 1525-7770.

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Official URL: https://doi.org/10.1080/15257770008045442

Abstract

2′,4′-Dideoxy-4′-methyleneuridine incorporated into oligodeoxynucleotides forms regular B-DNA duplexes as shown by Tm and CD measurements. Such oligomers are not cleaved by the DNA repair enzyme, UDG, which cleaves the glycosylic bond in dU but not in dT nor in dC nucleosides in single stranded and double stranded DNA. Differential binding of oligomers containing carbadU, 4′-thiodU, and dU residues to wild type and mutant UDG proteins identify an essential role for the furanose 4′-oxygen in recognition and cleavage of dU residues in DNA.

Metadata

Item Type:	Article
School:	Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
Research Centres and Institutes:	Innovation Management Research, Birkbeck Centre for
Depositing User:	Sarah Hall
Date Deposited:	14 May 2019 11:01
Last Modified:	02 Aug 2023 17:51
URI:	https://eprints.bbk.ac.uk/id/eprint/27522

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