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    The mechanism of DNA repair by uracil-DNA glycosylase: studies using nucleotide analogues

    Rösler A and Panayotou G and Hornby DP and Barlow T and Brown T and Pearl LH and Savva, Renos and Blackburn GM (2000) The mechanism of DNA repair by uracil-DNA glycosylase: studies using nucleotide analogues. Nucleosides, Nucleotides and Nucleic Acids 19 (10-12), pp. 1505-1516. ISSN 1525-7770.

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    Abstract

    2′,4′-Dideoxy-4′-methyleneuridine incorporated into oligodeoxynucleotides forms regular B-DNA duplexes as shown by Tm and CD measurements. Such oligomers are not cleaved by the DNA repair enzyme, UDG, which cleaves the glycosylic bond in dU but not in dT nor in dC nucleosides in single stranded and double stranded DNA. Differential binding of oligomers containing carbadU, 4′-thiodU, and dU residues to wild type and mutant UDG proteins identify an essential role for the furanose 4′-oxygen in recognition and cleavage of dU residues in DNA.

    Metadata

    Item Type: Article
    School: School of Science > Biological Sciences
    Research Centres and Institutes: Innovation Management Research, Birkbeck Centre for
    Depositing User: Sarah Hall
    Date Deposited: 14 May 2019 11:01
    Last Modified: 15 Oct 2019 09:03
    URI: https://eprints.bbk.ac.uk/id/eprint/27522

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