Browne, J.T. and Ng,, Y.-L. and Odlyha, Marianne and Gulablvala, K. and Bozec, L. (2020) Influence of root maturity or periodontal involvement on dentinal collagen changes following NaOCL irrigation: an ex-vivo study. International Endodontic Journal 53 (1), pp. 97-110. ISSN 0143-2885.
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Abstract
Abstract Aims: To refine FTIR protocol for detection of NaOCl-induced dentinal collagen changes using an ex- vivo irrigation model, and to apply it to determine the collagen change within 0.5mm of canal or root surfaces, with or without mature roots or periodontal involvement. Methodology: Extracted human roots were irrigated with control saline (n=3) or 5% NaOCl (n=3) and sectioned into transverse discs for FTIR analyses, 0.5mm from both the canal lumen and root surface, before and after surface-treatment with 17% EDTA. Amide I/phosphate and amide II/phosphate absorbance ratios were compared using the Wilcoxon sign rank test. Mature roots without periodontal involvement were irrigated with: saline (n=7), 5% NaOCl (n=7), or 5% NaOCl+17% EDTA (n=7); those with periodontal involvement (n=7) or immature roots (n=7) were irrigated with 5% NaOCl. Dentine discs were then prepared for FTIR analyses. The effects of irrigant/root-maturity/periodontal involvement were analysed using linear mixed models. Results: FTIR analyses of the irrigated samples revealed significant (P < 0.05) reduction in collagen bands near the canal lumen after NaOCl irrigation using surface-EDTA treated samples. Irrigation with test solutions resulted in significant (P < 0.0001) dentinal collagen changes in the mature roots, whilst those in the immature roots were significantly (P < 0.05) greater compared with the mature roots with or without periodontal involvement; but there were no difference between the latter groups. Conclusion: EDTA surface-treatment of polished dentine surfaces enhanced FTIR detection of NaOCl-induced collagen changes. Both root maturity and irrigation protocol influenced the ability of NaOCl to alter dentinal collagen up to 0.5mm from the canal lumen.
Metadata
| Item Type: | Article |
|---|---|
| Additional Information: | This is the peer reviewed version of the article, which has been published in final form at the link above. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Keyword(s) / Subject(s): | Sodium hypochlorite, dentine, collagen, FTIR, root maturity, periodontal disease |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Depositing User: | Marianne Odlyha |
| Date Deposited: | 12 Aug 2019 07:59 |
| Last Modified: | 13 Jun 2025 04:27 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/28488 |
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