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    Optimised oligonucleotide substrates to assay XPF ERCC1 nuclease activity for the discovery of DNA repair inhibitors

    Thomas, A.M. and Brolih, S. and McGouran, J. and El-Sagheer, A.H. and Ptchelkine, D. and Jones, M. and McDonald, Neil Q. and McHugh, P.J. and Brown, T. (2019) Optimised oligonucleotide substrates to assay XPF ERCC1 nuclease activity for the discovery of DNA repair inhibitors. Chemical Communications , ISSN 1359-7345. (In Press)

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    Abstract

    We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery

    Metadata

    Item Type: Article
    School: School of Science > Biological Sciences
    Research Centres and Institutes: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Neil McDonald
    Date Deposited: 16 Sep 2019 08:06
    Last Modified: 07 Jul 2020 04:28
    URI: https://eprints.bbk.ac.uk/id/eprint/28891

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