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    TEMPy2: a Python library with improved 3D electron microscopy density-fitting and validation workflows

    Cragnolini, Tristan and Sahota, Harpal and Joseph, Agnel Praveen and Sweeney, Aaron and Malhotra, Sony and Vasishtan, D. and Topf, Maya (2021) TEMPy2: a Python library with improved 3D electron microscopy density-fitting and validation workflows. Acta Crystallographica Section D: Structural Biology 77 (1), pp. 41-47. ISSN 2059-7983.

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    Abstract

    Structural determination of molecular complexes by cryo-EM requires large, often complex processing of the image data that are initially obtained. Here, TEMPy2, an update of the TEMPy package to process, optimize and assess cryo-EM maps and the structures fitted to them, is described. New optimization routines, comprehensive automated checks and workflows to perform these tasks are described.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): three-dimensional electron microscopy, model fitting, validation, fitting scores, model assessment, macromolecular complexes, TEMPy2.
    School: School of Science > Biological Sciences
    Depositing User: Administrator
    Date Deposited: 15 Jan 2021 13:01
    Last Modified: 16 Feb 2021 00:32
    URI: https://eprints.bbk.ac.uk/id/eprint/42601

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