Hutchings, Joshua and Stancheva, V. and Brown, N. and cheung, A. and Miller, E. and Zanetti, Giulia (2021) Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network. Nature Communications 12 , p. 2034. ISSN 2041-1723.
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Abstract
COPII mediates Endoplasmic Reticulum to Golgi trafficking of thousands of cargoes. Five essential proteins assemble into a two-layer architecture, with the inner layer thought to regulate coat assembly and cargo recruitment, and the outer coat forming cages assumed to scaffold membrane curvature. Here we visualise the complete, membrane-assembled COPII coat by cryo-electron tomography and subtomogram averaging, revealing the full network of interactions within and between coat layers. We demonstrate the physiological importance of these interactions using genetic and biochemical approaches. Mutagenesis reveals that the inner coat alone can provide membrane remodelling function, with organisational input from the outer coat. These functional roles for the inner and outer coats significantly move away from the current paradigm, which posits membrane curvature derives primarily from the outer coat. We suggest these interactions collectively contribute to coat organisation and membrane curvature, providing a structural framework to understand regulatory mechanisms of COPII trafficking and secretion.
Metadata
| Item Type: | Article |
|---|---|
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Research Centres and Institutes: | Structural Molecular Biology, Institute of (ISMB) |
| Depositing User: | Giulia Zanetti |
| Date Deposited: | 12 May 2021 14:06 |
| Last Modified: | 02 Aug 2023 18:08 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/43243 |
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