Telomerase RNA gene paralogs in plants - the usual pathway to unusual telomeres
Závodník, M. and Fajkus, P. and Franek, M. and Kopecký, D. and Garcia, S. and Dodsworth, Steven and Orejuela, A. and Kilar, A. and Ptáček, J. and Mátl, M. and Hýsková, A. and Fajkus, J. and Peška, V. (2023) Telomerase RNA gene paralogs in plants - the usual pathway to unusual telomeres. New Phytologist 239 (6), pp. 2353-2366. ISSN 0028-646X.
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New Phytologist - 2023 - Závodník - Telomerase RNA gene paralogs in plants the usual pathway to unusual telomeres.pdf - Published Version of Record Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (4MB) | Preview |
Abstract
Telomerase, telomeric DNA and associated proteins together represent a complex, finely tuned and functionally conserved mechanism that ensures genome integrity by protecting and maintaining chromosome ends. Changes in its components can threaten an organism's viability. Nevertheless, molecular innovation in telomere maintenance has occurred multiple times during eukaryote evolution, giving rise to species/taxa with unusual telomeric DNA sequences, telomerase components or telomerase-independent telomere maintenance. The central component of telomere maintenance machinery is telomerase RNA (TR) as it templates telomere DNA synthesis, its mutation can change telomere DNA and disrupt its recognition by telomere proteins, thereby leading to collapse of their end-protective and telomerase recruitment functions.Using a combination of bioinformatic and experimental approaches, we examine a plausible scenario of evolutionary changes in TR underlying telomere transitions.We identified plants harbouring multiple TR paralogs whose template regions could support the synthesis of diverse telomeres. In our hypothesis, formation of unusual telomeres is associated with the occurrence of TR paralogs that can accumulate mutations, and through their functional redundancy, allow for the adaptive evolution of the other telomere components.Experimental analyses of telomeres in the examined plants demonstrate evolutionary telomere transitions corresponding to TR paralogs with diverse template regions.
Metadata
| Item Type: | Article |
|---|---|
| Keyword(s) / Subject(s): | evolution, land plants, mechanism of telomere transitions, telomerase RNA paralogs, unusual telomere DNA |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Research Centres and Institutes: | Structural Molecular Biology, Institute of (ISMB) |
| Depositing User: | Steven Dodsworth |
| Date Deposited: | 27 Sep 2024 15:55 |
| Last Modified: | 27 Sep 2024 20:57 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/54013 |
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