Cauz-Santos, L.A. and Dodsworth, Steven and Samuel, R. and Christenhusz, M.J.M. and Patel, D. and Shittu, T. and Jakob, A. and Paun, O. and Chase, M.W. (2022) Genomic insights into recent species divergence in Nicotiana benthamiana and natural variation in Rdr1 gene controlling viral susceptibility. Plant Journal 111 (1), pp. 7-18. ISSN 0960-7412.
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The Plant Journal - 2022 - Cauz‐Santos - Genomic insights into recent species divergence in Nicotiana benthamiana and.pdf - Published Version of Record Available under License Creative Commons Attribution. Download (1MB) | Preview |
Abstract
One of the most commonly encountered and frequently cited laboratory organisms worldwide is classified taxonomically as Nicotiana benthamiana (Solanaceae), an accession of which, typically referred to as LAB, is renowned for its unique susceptibility to a wide range of plant viruses and hence capacity to be transformed using a variety of methods. This susceptibility is the result of an insertion and consequent loss of function in the RNA-dependent RNA polymerase 1 (Rdr1) gene. However, the origin and age of LAB and the evolution of N. benthamiana across its wide distribution in Australia remain relatively underexplored. Here, we have used multispecies coalescent methods on genome-wide single nucleotide polymorphisms (SNPs) to assess species limits, phylogenetic relationships and divergence times within N. benthamiana. Our results show that the previous taxonomic concept of this species in fact comprises five geographically, morphologically and genetically distinct species, one of which includes LAB. We provide clear evidence that LAB is closely related to accessions collected further north in the Northern Territory; this species split much earlier, c. 1.1 million years ago, from their common ancestor than the other four in this clade and is morphologically the most distinctive. We also found that the Rdr1 gene insertion is variable among accessions from the northern portions of the Northern Territory. Furthermore, this long-isolated species typically grows in sheltered sites in subtropical/tropical monsoon areas of northern Australia, contradicting the previously advanced hypothesis that this species is an extremophile that has traded viral resistance for precocious development.
Metadata
| Item Type: | Article |
|---|---|
| Keyword(s) / Subject(s): | coalescent, LAB benthamiana, phylogenomics, RADseq, Rdr1, single-digest restriction-site associated DNA (sdRAD), virus susceptibility |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Research Centres and Institutes: | Structural Molecular Biology, Institute of (ISMB) |
| Depositing User: | Steven Dodsworth |
| Date Deposited: | 27 Sep 2024 05:05 |
| Last Modified: | 27 Sep 2024 05:25 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/54021 |
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