Menendez-Gil, P. and Veleva, D. and VIRGO, Mollie and Zhang, J. and Ramalhete, R. and Ho, Brian T. (2024) Modulation of Vibrio cholerae gene expression through conjugative delivery of engineered regulatory small RNAs. Journal of Bacteriology , ISSN 0021-9193.
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Abstract
The increase in antibiotic resistance in bacteria has prompted the efforts in developing new alternative strategies for pathogenic bacteria. We explored the feasibility of targeting Vibrio cholerae by neutralizing bacterial cellular processes rather than outright killing the pathogen. We investigated the efficacy of delivering engineered regulatory small RNAs (sRNAs) to modulate gene expression through DNA conjugation. As a proof of concept, we engineered several sRNAs targeting the type VI secretion system (T6SS), several of which were able to successfully knockdown the T6SS activity at different degrees. Using the same strategy, we modulated exopolysaccharide production and motility. Lastly, we delivered an sRNA targeting T6SS into V. cholerae via conjugation and observed a rapid knockdown of the T6SS activity. Coupling conjugation with engineered sRNAs represents a novel way of modulating gene expression in V. cholerae opening the door for the development of novel prophylactic and therapeutic applications.
Metadata
| Item Type: | Article |
|---|---|
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Research Centres and Institutes: | Structural Molecular Biology, Institute of (ISMB) |
| Depositing User: | Brian Ho |
| Date Deposited: | 22 Oct 2024 12:52 |
| Last Modified: | 22 Oct 2024 20:43 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/54426 |
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