Melo, I.S. and Moretini, A. and Cassiolato, A.M.R. and Nicklin, Jane (2011) Development of mutants of Coniothyrium Minitans with improved efficiency for control of Sclerotinia Sclerotiorum. Journal of Plant Protection Research 51 (2), pp. 179-183. ISSN 1427-4345.
Abstract
Coniothyrium minitans (CM) is hyperparasitic to Sclerotinia sclerotiorum (SS), a pathogen of many economically important crops. In this paper, we describe the isolation of improved mutants of CM, using a UV - irradiation regime, with altered chitinase production and tolerance to high concentration of iprodione, which are effective against SS. Three out of the 59 mutants obtained inhibited the mycelial growth of CM. Infectivity of sclerotia by the new mutants was assayed by the plant-tissue-based system using carrot segments. More than 80% of sclerotia were colonized by the mutants and the wild-type CM. The mutant strains retained ability to produce significant amounts of chitinase. The mutants differed from their wild-type strain in appearance, morphology and sporulation. In conclusion, the results presented here provide evidence that the new biotypes of C. minitans are effective in controlling S. sclerotiorum.
Metadata
| Item Type: | Article |
|---|---|
| Keyword(s) / Subject(s): | Coniothyrium minitans, Sclerotinia sclerotiorum, iprodione, resistance, biological control, antibiotic production |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Research Centres and Institutes: | Structural Molecular Biology, Institute of (ISMB) |
| Depositing User: | Administrator |
| Date Deposited: | 19 Dec 2012 17:55 |
| Last Modified: | 02 Aug 2023 17:00 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/5724 |
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