Daviter, Tina and Fronzes, Remi (2013) Protein sample characterization. In: Williams, Mark A. and Daviter, Tina (eds.) Protein–Ligand Interactions: Fundamentals. Methods in Molecular Biology I 1008. Berlin, Germany: Springer, pp. 35-62. ISBN 9781627033978.
Abstract
Most biophysical experiments require protein samples of high quality and accurately determined concentration. This chapter attempts to compile basic information on the most common methods to assess the purity, dispersity, and stability of protein samples. It also reminds of methods to measure protein concentration and of their limits. The idea is to make aware and remind of the range of methods available and of commonly overlooked pitfalls. The aim is to enable experimenters to fully characterize their preparations of soluble or membrane proteins and gain reliable and reproducible results from their experimental work.
Metadata
| Item Type: | Book Section |
|---|---|
| Additional Information: | Series ISSN: 1064-3745 |
| Keyword(s) / Subject(s): | Purity, Concentration, Solubility, Homogeneity, Electrophoresis, Absorbance |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Depositing User: | Administrator |
| Date Deposited: | 29 Jan 2014 13:25 |
| Last Modified: | 02 Aug 2023 17:09 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/9109 |
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