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    Protein sample characterization

    Daviter, Tina and Fronzes, Remi (2013) Protein sample characterization. In: Williams, Mark A. and Daviter, Tina (eds.) Protein–Ligand Interactions: Fundamentals. Methods in Molecular Biology I 1008. Berlin, Germany: Springer, pp. 35-62. ISBN 9781627033978.

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    Abstract

    Most biophysical experiments require protein samples of high quality and accurately determined concentration. This chapter attempts to compile basic information on the most common methods to assess the purity, dispersity, and stability of protein samples. It also reminds of methods to measure protein concentration and of their limits. The idea is to make aware and remind of the range of methods available and of commonly overlooked pitfalls. The aim is to enable experimenters to fully characterize their preparations of soluble or membrane proteins and gain reliable and reproducible results from their experimental work.

    Metadata

    Item Type: Book Section
    Additional Information: Series ISSN: 1064-3745
    Keyword(s) / Subject(s): Purity, Concentration, Solubility, Homogeneity, Electrophoresis, Absorbance
    School: School of Science > Biological Sciences
    Depositing User: Administrator
    Date Deposited: 29 Jan 2014 13:25
    Last Modified: 28 Jul 2020 09:44
    URI: https://eprints.bbk.ac.uk/id/eprint/9109

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