Lowe, P.N. and Vaughan, Cara K. and Daviter, Tina (2013) Measurement of protein–ligand complex formation. In: Williams, Mark A. and Daviter, Tina (eds.) Protein–Ligand Interactions: Fundamentals. Methods in Molecular Biology I 1008. Berlin, Germany: Springer, pp. 63-99. ISBN 9781627033978.
Abstract
Experimental approaches to detect, measure, and quantify protein–ligand binding, along with their theoretical bases, are described. A range of methods for detection of protein–ligand interactions is summarized. Specific protocols are provided for a nonequilibrium procedure pull-down assay, for an equilibrium direct binding method and its modification into a competition-based measurement and for steady-state measurements based on the effects of ligands on enzyme catalysis.
Metadata
| Item Type: | Book Section |
|---|---|
| Additional Information: | Series ISSN: 1064-3745 |
| Keyword(s) / Subject(s): | Complex formation, Equilibrium binding, Kinetics, Affinity, Stoichiometry, Interaction, Pull-down assay, Competition, Steady state |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Research Centres and Institutes: | Structural Molecular Biology, Institute of (ISMB) |
| Depositing User: | Administrator |
| Date Deposited: | 29 Jan 2014 13:29 |
| Last Modified: | 02 Aug 2023 17:09 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/9110 |
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