Measurement of protein–ligand complex formation

Lowe, P.N. and Vaughan, Cara K. and Daviter, Tina (2013) Measurement of protein–ligand complex formation. In: Williams, Mark A. and Daviter, Tina (eds.) Protein–Ligand Interactions: Fundamentals. Methods in Molecular Biology I 1008. Berlin, Germany: Springer, pp. 63-99. ISBN 9781627033978.

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Official URL: http://dx.doi.org/10.1007/978-1-62703-398-5_3

Abstract

Experimental approaches to detect, measure, and quantify protein–ligand binding, along with their theoretical bases, are described. A range of methods for detection of protein–ligand interactions is summarized. Specific protocols are provided for a nonequilibrium procedure pull-down assay, for an equilibrium direct binding method and its modification into a competition-based measurement and for steady-state measurements based on the effects of ligands on enzyme catalysis.

Metadata

Item Type:	Book Section
Additional Information:	Series ISSN: 1064-3745
Keyword(s) / Subject(s):	Complex formation, Equilibrium binding, Kinetics, Affinity, Stoichiometry, Interaction, Pull-down assay, Competition, Steady state
School:	Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
Research Centres and Institutes:	Structural Molecular Biology, Institute of (ISMB)
Depositing User:	Administrator
Date Deposited:	29 Jan 2014 13:29
Last Modified:	02 Aug 2023 17:09
URI:	https://eprints.bbk.ac.uk/id/eprint/9110

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