BIROn - Birkbeck Institutional Research Online

    Measurement of protein–ligand complex formation

    Lowe, P.N. and Vaughan, Cara K. and Daviter, Tina (2013) Measurement of protein–ligand complex formation. In: Williams, Mark A. and Daviter, Tina (eds.) Protein–Ligand Interactions: Fundamentals. Methods in Molecular Biology I 1008. Berlin, Germany: Springer, pp. 63-99. ISBN 9781627033978.

    Full text not available from this repository.

    Abstract

    Experimental approaches to detect, measure, and quantify protein–ligand binding, along with their theoretical bases, are described. A range of methods for detection of protein–ligand interactions is summarized. Specific protocols are provided for a nonequilibrium procedure pull-down assay, for an equilibrium direct binding method and its modification into a competition-based measurement and for steady-state measurements based on the effects of ligands on enzyme catalysis.

    Metadata

    Item Type: Book Section
    Additional Information: Series ISSN: 1064-3745
    Keyword(s) / Subject(s): Complex formation, Equilibrium binding, Kinetics, Affinity, Stoichiometry, Interaction, Pull-down assay, Competition, Steady state
    School: School of Science > Biological Sciences
    Research Centres and Institutes: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 29 Jan 2014 13:29
    Last Modified: 28 Jul 2020 09:44
    URI: https://eprints.bbk.ac.uk/id/eprint/9110

    Statistics

    Downloads
    Activity Overview
    0Downloads
    167Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item