Nans, Andrea and Saibil, Helen R. and Hayward, Richard (2014) Pathogen-host reorganization during Chlamydia invasion revealed by cryo-electron tomography. Cellular Microbiology 16 (10), pp. 1457-1472. ISSN 1462-5814.
![[img]](https://eprints.bbk.ac.uk/style/images/fileicons/text.png) |
Text
9701.pdf - Author's Accepted Manuscript Restricted to Repository staff only Available under License Creative Commons Attribution. Download (2MB) |
|
|
Text
9701A.pdf - Published Version of Record Available under License Creative Commons Attribution. Download (2MB) | Preview |
Abstract
Invasion of host cells is a key early event during bacterial infection, but the underlying pathogen-host interactions are yet to be fully visualised in three-dimensional detail. We have captured snapshots of the early stages of bacterial-mediated endocytosis in situ by exploiting the small size of chlamydial elementary bodies (EBs) for whole cell cryo-electron tomography. Chlamydiae are obligate intracellular bacteria that infect eukaryotic cells and cause sexually transmitted infections and trachoma, the leading cause of preventable blindness. We demonstrate that Chlamydia trachomatis LGV2 EBs are intrinsically polarised. One pole is characterised by a tubular inner membrane invagination, while the other exhibits asymmetric periplasmic expansion to accommodate an array of type III secretion systems (T3SSs). Strikingly, EBs orient with their T3SS-containing pole facing target cells, enabling the T3SSs to directly contact the cellular plasma membrane. This contact induces enveloping macropinosomes, actin-rich filopodia and phagocytic cups to zipper tightly around the internalising bacteria. Once encapsulated into tight early vacuoles, EB polarity and the T3SSs are lost. Our findings reveal previously undescribed structural transitions in both pathogen and host during the initial steps of chlamydial invasion.
Metadata
| Item Type: | Article |
|---|---|
| Keyword(s) / Subject(s): | Chlamydia, electron tomography, host-pathogen interactions, type III secretion |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Research Centres and Institutes: | Structural Molecular Biology, Institute of (ISMB) |
| Depositing User: | Administrator |
| Date Deposited: | 12 May 2014 11:44 |
| Last Modified: | 02 Aug 2023 17:10 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/9701 |
Statistics
Additional statistics are available via IRStats2.
Archive Staff Only (login required)
